Can you integrate TCR sequencing data with other single-cell data, like gene expression?

Yes, our platform is compatible with multi-modal single-cell analysis. We can provide paired TCR and 5' gene expression data from the same single cells, allowing you to link T-cell clonotype with its functional phenotype. Please inquire about this service.

High-Accuracy De Novo T Cell Receptor (TCR) Sequencing Services

Q: Can you perform TCR sequencing on non-human samples?

Absolutely. Our de novo approach is ideal for analyzing the TCR repertoire of any species, including mice, non-human primates, and other model organisms, as it does not depend on a pre-existing, annotated reference genome.

Background Platform Workflow Advantage Application Related Services FAQ Online Inquiry

Unraveling the complexity of the adaptive immune response requires a profound understanding of the T-cell receptor (TCR) repertoire. At Creative Biolabs, we harness cutting-edge technology to provide industry-leading De Novo T Cell Receptor (TCR) Sequencing services. With over 20 years of expertise in immunology and sequencing, we empower researchers to discover novel TCRs, characterize clonal dynamics, and identify antigen-specific T cells with unparalleled accuracy and depth. Our platform is engineered for discovery, enabling the complete and unbiased characterization of TCR alpha and beta chains, even from novel species or in contexts where no reference genome exists.

Discuss Your Project Today

The Imperative of De Novo Sequencing for TCR Analysis

The T-cell receptor is a heterodimeric protein complex on the surface of T lymphocytes responsible for recognizing specific antigens presented by major histocompatibility complex (MHC) molecules. The immense diversity of the TCR repertoire, generated by V(D)J recombination, allows the immune system to recognize a virtually limitless array of foreign and self-antigens.

De Novo T Cell Receptor (TCR) Sequencing is the definitive method for characterizing this diversity without reliance on existing reference databases for the variable regions. This is critical for:

Discovering Novel Clonotypes: Identifying unique TCRs associated with specific diseases, such as cancer neoantigens or novel viral epitopes.
Accurate Paired-Chain Identification: True de novo approaches ensure the precise pairing of alpha and beta chains that form a functional receptor.
Cross-Species Research: Studying the immune repertoires of non-model organisms where comprehensive reference databases are unavailable.
Understanding Atypical Recombination: Characterizing TCRs that arise from non-canonical V(D)J recombination events, which would be missed by alignment-based methods.

Fig. 1 T-cell therapy. (Creative Biolabs Authorized). Fig. 1 TCR T-cell therapy.

At Creative Biolabs, we have perfected the art of De Novo Sequencing, applying its principles to provide the most accurate and comprehensive view of your T-cell populations.

Our Advanced De Novo TCR Sequencing Platform

To deliver best-in-class results, our De Novo T Cell Receptor (TCR) Sequencing service integrates multiple state-of-the-art technologies.

5' RACE for Full-Length V(D)J Coverage: Our methodology employs 5' Rapid Amplification of cDNA Ends (RACE), an unbiased approach that captures the entire V(D)J segment of both TCR chains. This is superior to multiplex PCR methods, which can introduce amplification bias.
Next-Generation Sequencing (NGS): Libraries are sequenced on industry-leading platforms, generating millions of high-quality reads per sample. This depth allows for the detection of even extremely rare T-cell clones, which are often biologically significant.
Proprietary De Novo Assembly & Bioinformatics: This is the core of our service. Our powerful bioinformatics pipeline assembles TCR sequences without aligning to a reference V(D)J database. It then annotates the assembled contigs, identifies CDR3 regions, and performs in-depth repertoire analysis.

De Novo TCR Sequencing Service Workflow: From Sample to Insight

We offer a comprehensive, end-to-end service managed by our dedicated team of Ph.D.-level scientists.

Project Consultation: Our experts work with you to understand your research goals and design the optimal experimental strategy.
Sample Preparation: We accept a wide range of sample types, including PBMCs, isolated T cells, tumor-infiltrating lymphocytes (TILs), and fresh-frozen tissue. We perform rigorous QC at every step.
Library Construction: Using our advanced platform, we prepare 5' RACE-based, paired-chain TCR libraries.
Deep Sequencing: Libraries are sequenced to your specified depth on our platforms.
De Novo Bioinformatics Analysis: Our proprietary pipeline performs error correction, de novo assembly, V(D)J annotation, clonotyping, and comprehensive repertoire characterization.
Data Delivery: You receive a detailed report including raw data, assembled TCR sequences, clonotype tables, and publication-ready figures. Our scientists are available for post-delivery support to help you interpret the results.

Key Features and Advantages of Our De Novo TCR Sequencing Service

Feature	Creative Biolabs' Advantage	Your Research Benefit
*True De Novo* Sequencing**	Proprietary algorithms assemble TCR sequences without reference database bias.	Discovery Power: Confidently identify novel and rare TCR clonotypes missed by other methods.
Paired Chains	Captures natively paired TCR αβ chains from individual cells.	Functional Accuracy: Eliminate ambiguity; know the exact receptor composition for downstream functional studies.
Unbiased Amplification	5' RACE-based approach ensures uniform amplification across all V-genes.	Quantitative Precision: Obtain a more accurate representation of the true clonotype frequencies in your sample.
High-Throughput & Depth	Leverage sequencing for deep profiling of complex repertoires.	High Sensitivity: Detect rare, antigen-specific T-cell clones critical in disease and immunity.
Comprehensive Bioinformatics	Includes clonotyping, diversity metrics, V(D)J usage, and advanced visualizations.	Actionable Insights: Receive a complete, publication-ready analysis report, saving you time and resources.
Expert Support	Direct access to our team of immunology and sequencing experts from start to finish.	Project Success: Ensure your project is designed for success and that you can fully interpret your complex data.

Applications of De Novo TCR Sequencing

Our services provide critical insights across numerous research fields, accelerating discovery and therapeutic development.

Immuno-Oncology: Characterize the T-cell response to tumors and immunotherapies.

Identify Tumor-Specific Clonotypes: Discover TCRs from tumor-infiltrating lymphocytes (TILs) that recognize neoantigens.
Monitor Immunotherapy Response: Track the expansion and contraction of cancer-reactive T-cell clones in patients treated with checkpoint inhibitors, CAR-T cells, or cancer vaccines.
TCR-T Cell Therapy Development: Identify potent anti-tumor TCRs for engineering next-generation T-cell therapies. Our De Novo Antibody Sequencing expertise complements this for comprehensive therapeutic development.

Infectious Diseases: Profile the T-cell response to viral, bacterial, and parasitic infections.

Discover Pathogen-Specific TCRs: Identify the dominant T-cell clonotypes that respond to specific pathogen epitopes.
Vaccine Development & Efficacy: Evaluate the diversity and magnitude of the T-cell response elicited by novel vaccines. Track memory T-cell formation and persistence.

Autoimmune Disorders: Investigate the role of self-reactive T cells in autoimmune diseases.

Characterize Autoreactive Clonotypes: Identify and track pathogenic T-cell clones in diseases like rheumatoid arthritis, multiple sclerosis, and type 1 diabetes.
Mechanism of Action Studies: Understand how therapeutics modulate the autoreactive T Cell Receptor (TCR) repertoire.

Allergy and Transplantation: Analyze T-cell responses in allergic reactions and graft-versus-host disease (GVHD).

Identify Allergen-Specific T Cells: Characterize the TCRs involved in hypersensitivity reactions.
Monitor Alloreactivity: Track the T-cell clones responsible for transplant rejection or GVHD.

Explore Our Comprehensive Services

To further support your research, Creative Biolabs offers a suite of antibody sequencing related services:

Unlock a deeper understanding of the adaptive immune system with Creative Biolabs. Contact our team of experts to discuss your project and learn how our De Novo T Cell Receptor (TCR) Sequencing services can help you achieve your research goals.

Contact Us Today for a Free Quote!

Frequently Asked Questions (FAQs)

Q: What is the difference between your de novo service and standard, reference-based TCR sequencing?

A: Standard methods rely on aligning sequencing reads to a known V(D)J gene database. Our De Novo TCR Sequencing assembles the full variable region sequence directly from the reads, allowing for the discovery of novel alleles, unannotated genes, and unique recombination events that alignment-based methods would miss.

Q: Can you perform TCR sequencing on non-human samples?

A: Absolutely. Our de novo approach is ideal for analyzing the TCR repertoire of any species, including mice, non-human primates, and other model organisms, as it does not depend on a pre-existing, annotated reference genome.

Q: What is the typical turnaround time for a project?

A: Turnaround time varies depending on the project scope and number of samples, but typically ranges from 4-6 weeks from sample receipt to data delivery. We will provide a specific timeline in your project proposal.

Reference

Li, Yilong, et al. "Circulating T-cell receptor repertoire for cancer early detection." NPJ Precision Oncology 9.1 (2025): 245. https://doi.org/10.1038/s41698-025-01036-y

×

Online Inquiry

All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.